Formalin-fixed paraffin embedded tissue (FFPE)
5-10 10µm sections of FFPE tissue in a sterile cup with stained H&E of same block.
If tumor percentage is less than 10% of total nuclei, macrodissection is required, send 5-10 10µm thickness unstained slides with stained H&E of same block.
Storage and stability information:
Once per week
Real-time Polymerase Chain Reaction (PCR)
No EGFR mutation detected
Somatic mutations in the epidermal growth factor receptor (EGFR) gene have been associated with non-small cell lung cancer (NSCLC) patients’ responsiveness to tyrosine kinase inhibitors (TKI) (gefinitib, erlotinib). However, while some mutations have a sensitizing effect, other mutations are linked to TKI resistance.