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Test name:
FISH, HER2 amplification

Order name:
HER2 by FISH request

Specimen requirements:
Formalin fixed paraffin-embedded tissue (FFPE)

Minimum volume:
FFPE – 2 unstained 4µm thick sections on positively charged slides plus 1 stained H&E slide of the same block

Storage and stability information:
FFPE at room temperature indefinitely.

Test performed:
Twice per week

Fluorescence in situ hybridization

Reference range:
See lab report

Clinical significance:
This test can be used as a prognostic indicator for patients with both node-positive or node-negative breast cancer; to guide therapy, as patients with HER2 amplification may be candidates for Herceptin therapy; and to confirm the presence of HER2 amplification in cases with 2+ (low-level) or 3+ (high-level) HER2 overexpression by immunohistochemistry.

CPT codes:
88368 x 2

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