Flow Cytometry

Leukemia/Lymphoma Immunophenotyping

Immunophenotyping can help diagnose and classify blood cell cancers (leukemias and lymphomas). It may be ordered as a follow-up test when a white cell count differential shows an increased number of lymphocytes, the presence of immature white cells, an idiopathic change in the red cell indices or when there is a significant increase or decrease in the number of platelets (thrombocytosis or thrombocytopenia). Testing is most often performed on blood and/or bone marrow samples, but may also be done on body fluids or other biopsy tissue samples.

Immunophenotyping by Flow Cytometry is useful to:

• Evaluate lymphocytoses of unknown etiology
• Identify B- and T-cell lymphoproliferative disorders
• Distinguish acute lymphoblastic leukemia (ALL) from acute myeloid leukemia (AML)
• Subtype ALL
• Distinguish reactive lymphocytes  from malignant lymphoma
• Distinguish between malignant lymphoma and acute leukemia
• Phenotype subclassification of B- and T-cell chronic lymphoproliferative disorders
• Recognize AML with minimal morphologic evidence of differentiation

Lymphocyte Enumeration

The BayCare lymphocyte enumeration panel contains:

An abbreviated CD4/CD8 panel is also available.

DNA Ploidy and S-Phase Cell Cycle Analysis

DNA ploidy and cell cycle analysis is a rapid and efficient way to evaluate the DNA content (ploidy) and proliferative activity (cell cycle/S-phase fraction) of cells. By staining the DNA with a fluorescent dye, flow cytometry can measure the dye fluorescence in many individual cells, and the data can be analyzed for ploidy (diploid/normal content or aneuploid/abnormal content) and proliferative activity.

For more information, please call (800) 324-7853.