CCND1/IGH t(11;14) by FISH

Test name:
FISH, CCND1/IGH t(11;14)

Order name:
t(11;14) by FISH request

Specimen requirements:
Sodium heparin peripheral blood or bone marrow. Formalin fixed paraffin-embedded tissue (FFPE)

Minimum volume:
1mL blood or bone marrow. FFPE – 2 unstained 4µm thick sections on positively charged slides plus 1 stained H&E slide of the same block

Storage and stability information:
Room temperature 3 days for blood and bone marrow. FFPE at room temperature indefinitely.

Test performed:
Twice per week

Methodology:
Fluorescence in situ hybridization

Reference range:
See lab report

Clinical significance:
The t(11;14) translocation leads to juxtaposition of the Immunoglobulin Heavy Chain loci with the CCND1 (Cyclin D1) gene and is found in almost all cases of mantle cell lymphoma.  The t(11;14) is not specific for mantle cell lymphoma however, as it can be identified in other B-cell neoplasms, including plasma cell myeloma.

CPT codes:
88368 x 2